Coding
Part:BBa_K4410002
Designed by: Ruyi Shi Group: iGEM22_Worldshaper-HZBIOX (2022-09-19)
argR
This part is argR gene. argR gene is from the genome of E.coli Nissle 1917. It encodes argR protein, an arginine repressor causing negative inhibition of arginine biosynthesis in E coli bacteria. In our project, we knocked down argR gene by replacing argR gene with KanR gene, in order to prevent feedback inhibition of arginine synthesizing pathway from accumulated arginine.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 27
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 27
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 27
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 27
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |